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When working with IEM, i5 sequences have to be entered into these software program during the forward orientation in all instances and for all instrument kinds.

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When demultiplexing, the ultimate sample sheet presented on the FASTQ generation software really should provide the i5 index in the correct orientation for that System. Even so, most Illumina sample sheet era software handles this quickly.

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Established D Index Adapters Consult with Index two (i5) Orientation for more information on how to enter i5 bases to the sample sheet in forward or reverse complement orientation.

This means that these software Focus on the assumption that the i5 sequence is usually inside the forward orientation and may mechanically generate the reverse complement of the sequences delivered for the suitable instrument type.

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